• SLIDE (Sparse Linear modeling of RNA-Seq data for Isoform Discovery and abundance Estimation)
  • Citation: Li, J.J., Jiang, C.-R., Brown, B.J., Huang, H., and Bickel, P.J. (2011). Sparse linear modeling of RNA-seq data for isoform discovery and abundance estimation. Proc Natl Acad Sci. USA 108(50):19867-19872.
  • Important note: SLIDE is compatible with RNA-seq .bam files mapped by TopHat and TopHat2.
  • (Updates on Jan 30th, 2018 -- Several bugs have been fixed for RNA-seq .bam files with more than one read lengths.)
  • (Updates on May 7th, 2012 -- A feature was added for estimating the annotated isoform abundance without doing isoform discovery.)
  • (Updates on Apr 18th, 2012 -- A feature was added for removing erroneously mapped reads; a bug of multiprocessing was fixed.)
  • (Updates on Apr 5th, 2012 -- A feature was added for handling single-end RNA-Seq reads or a mixture of single-end and paired-end reads.)

• NMFP (Non-negative Matrix Factorization based Preselection)
  • Please cite the following paper in any research that uses this software package
    • Ye, Y. and Li, J.J. (2016). NMFP: a non-negative matrix factorization based preselection method to increase accuracy of identifying mRNA isoforms from RNA-seq data. BMC Genomics 17(Supp 1):11.
• MSIQ (joint modeling of Multiple RNA-seq Samples for accurate Isoform Quantification)
  • Citation: Li, W.V., Zhao, A., Zhang, S., and Li, J.J. (2018). MSIQ: joint modeling of multiple RNA-seq samples for accurate isoform quantification. Annals of Applied Statistics 12(1):510-539.
• AIDE (annotation-assisted isoform discovery)
  • Citation: Li, W.V., Li, S., Tong, X., Deng, L., Shi, H., and Li, J.J. (2019). AIDE: annotation-assisted isoform discovery with high precision. Genome Research 29:2056-2072.

Single-cell RNA-seq

• scImpute (imputation method for single-cell RNA-Seq data)
  • Citation: Li, W.V. and Li, J.J. (2018). An accurate and robust imputation method scImpute for single-cell RNA-seq data. Nature Communications 9:997.
• scDesign (a statistical simulator for rational scRNA-seq experimental design)
  • Citation: Li, W.V. and Li, J.J. (2019). A statistical simulator scDesign for rational scRNA-seq experimental design. Bioinformatics 35(14):i41–i50.
• scDesign2 (a simulator that generates single-cell gene expression counts with gene correlations captured)
  • Citation: Sun, T., Song, D., Li, W.V., and Li, J.J. (2021). scDesign2: a transparent simulator that generates high-fidelity single-cell gene expression count data with gene correlations captured. Genome Biology 22:163.
• PseudotimeDE (inference of differential gene expression along cell pseudotime)
  • Citation: Song, D. and Li, J.J. (2021). PseudotimeDE: inference of differential gene expression along cell pseudotime with well-calibrated p-values from single-cell RNA sequencing data. Genome Biology 22:124.
• scPNMF (single-cell projective non-negative matrix factorization)
  • Citation: Song, D., Li, K.A., Hemminger, Z., Wollman, R., and Li, J.J. (2021). scPNMF: sparse gene encoding of single cells to facilitate gene selection for targeted gene profiling. Bioinformatics in press.

Comparative genomics

• TROM (TRanscriptome Overlap Measure)
  • Citation: Li, W.V., Chen, Y., and Li, J.J. (2017). TROM: a testing-based method for finding transcriptomic similarity of biological samples. Statistics in Biosciences 9(1):105-136.
• EPOM (EPigenome Overlap Measure)
  • Citation: Li, W.V., Razaee, Z.S., and Li, J.J. (2016). Epigenome overlap measure (EPOM) for comparing tissue/cell types based on chromatin states. BMC Genomics 17(Supp 1):10.
• EpiAlign (an alignment-based bioinformatic tool for comparing chromatin state sequences)
  • Citation: Ge, X., Zhang, H., Xie, L., Li, W.V., Kwon, S.B., and Li, J.J. (2019). EpiAlign: an alignment-based bioinformatic tool for comparing chromatin state sequences. Nucleic Acids Research gkz287.

• BiTSC (bipartite tight spectral clustering)
  • Citation: Sun, Y.E., Zhou, H.J., and Li, J.J. (2020). Bipartite tight spectral clustering (BiTSC) algorithm for identifying conserved gene co-clusters in two species. Bioinformatics btaa741.

Microbiomics

• mbImpute (imputation method for microbiome data)
  • Citation: Jiang, R., Li, W.V., and Li, J.J. (2021). mbImpute: an accurate and robust imputation method for microbiome data. Genome Biology accepted after minor revision.

Classification

• NPROC (Neyman-Pearson (NP) classification algorithms and NP receiver operating characteristics (NP-ROC))
  • Citation: Tong, X., Feng, Y., and Li, J.J. (2018). Neyman-Pearson (NP) classification algorithms and NP receiver operating characteristics (NP-ROC). Science Advances 4(2):eaao1659.
• frc (feature ranking for classification)
  • Citation: Li, J.J., Chen, Y., and Tong, X. (2021). A flexible model-free prediction-based framework for feature ranking. Journal of Machine Learning Research 22:1-54.

Association measure

• gR2 (generalized R squares measures)
  • Citation: Li, J.J., Tong, X., and Bickel, P.J. (2019). Generalized R2 measures for a mixture of bivariate linear dependences. arXiv:1811.09965.

High-dimensional model inference

• HDCI (high-dimensional linear model coefficient confidence interval)
  • Citation: Liu, H., Xu, X., and Li, J.J. (2020). A bootstrap lasso + partial ridge method to construct confidence intervals for parameters in high-dimensional sparse linear models. Statistica Sinica 30:1333-1355.

False discovery rate control

• Clipper (p-value-free FDR control in high-throughput genomics data analysis)
  • Citation: Ge, X., Chen, Y.E., Song, D., McDermott, M., Woyshner, K., Manousopoulou, A., Wang, L.D., Li, W., and Li, J.J. (2020). Clipper: p-value-free FDR control on high-throughput data from two conditions. bioRxiv. doi: https://doi.org/10.1101/2020.11.19.390773

Data

• Estimates of D. melanogaster and C. elegans gene expression in different developmental stages, tissues and cells (in FPKM units)
  • D. melanogaster gene expression estimates in 30 fly developmental stages (download)
  • D. melanogaster gene expression estimates in 29 fly tissues and 19 fly cell lines (download)
  • C. elegans gene expression estimates in 35 worm developmental stages (download)
  • C. elegans gene expression estimates in 4 worm tissues and 14 worm dissected cells (download)
  • Please cite the following paper in any research that uses the above data
    • Li, J.J., Huang, H., Bickel, P.B., and Brenner, S.E. (2014). Comparison of D. melanogaster and C. elegans developmental stages, tissues, and cells by modENCODE RNA-seq data. Genome Research 24(7):1086-1101.
  • For more details about the data, please refer to the section "Estimating gene expression in developmental stages and tissues/cells" in the Methods of the above paper ([html] [pdf]).

• Associated promoter and enhancer regions identified based on signals of three histone modification marks (H3K4me1, H3K4me3 and H3K27ac) in 16 human tissue and cell types (download)

• Estimates of gene expression (FPKM) in various cell and tissue types from human, chimpanzee, bonobo and mouse
  • Expression estimates of protein-coding genes in human (download)
  • Expression estimates of protein-coding genes in chimpanzee (download)
  • Expression estimates of protein-coding genes in bonobo (download)
  • Expression estimates of protein-coding genes in mouse (download)
  • Expression estimates of protein-coding genes in pig (download)
  • Expression estimates of long non-coding RNAs in human (download)
  • Expression estimates of long non-coding RNAs in chimpanzee (download)
  • Expression estimates of long non-coding RNAs in bonobo (download)
  • Expression estimates of long non-coding RNAs in mouse (download)
  • Please cite the following paper in any research that uses the above data
    • Yang et al. Large-scale mapping of mammalian transcriptomes identifies conserved genes associated with different cell states. Nucleic Acids Research 45(4):1657–1672.
  • For more details about the data, please refer to the section "RNA-seq data collection and processing" in the Methods of the above paper ([html] [pdf]).

• Data for the R package Clipper (download)