• SLIDE (Sparse Linear modeling of RNA-Seq data for Isoform Discovery and abundance Estimation)
  • Citation: Li, J.J., Jiang, C.-R., Brown, B.J., Huang, H., and Bickel, P.J. (2011). Sparse linear modeling of RNA-seq data for isoform discovery and abundance estimation. Proc Natl Acad Sci. USA 108(50):19867-19872.
  • Important note: SLIDE is compatible with RNA-seq .bam files mapped by TopHat and TopHat2.
  • (Updates on Jan 30th, 2018 -- Several bugs have been fixed for RNA-seq .bam files with more than one read lengths.)
  • (Updates on May 7th, 2012 -- A feature was added for estimating the annotated isoform abundance without doing isoform discovery.)
  • (Updates on Apr 18th, 2012 -- A feature was added for removing erroneously mapped reads; a bug of multiprocessing was fixed.)
  • (Updates on Apr 5th, 2012 -- A feature was added for handling single-end RNA-Seq reads or a mixture of single-end and paired-end reads.)

• NMFP (Non-negative Matrix Factorization based Preselection)
  • Please cite the following paper in any research that uses this software package
    • Ye, Y. and Li, J.J. (2016). NMFP: a non-negative matrix factorization based preselection method to increase accuracy of identifying mRNA isoforms from RNA-seq data. BMC Genomics 17(Supp 1):11.
• TROM (TRanscriptome Overlap Measure)
  • Citation: Li, W.V., Chen, Y., and Li, J.J. (2017). TROM: a testing-based method for finding transcriptomic similarity of biological samples. Statistics in Biosciences 9(1):105-136.
• EPOM (EPigenome Overlap Measure)
  • Citation: Li, W.V., Razaee, Z.S., and Li, J.J. (2016). Epigenome overlap measure (EPOM) for comparing tissue/cell types based on chromatin states. BMC Genomics 17(Supp 1):10.
• scImpute (accurate and robust imputation for single-cell RNA-Seq data)
  • Citation: Li, W.V. and Li, J.J. (2018). An accurate and robust imputation method scImpute for single-cell RNA-seq data. Nature Communications 9:997.
• NPROC (Neyman-Pearson (NP) classification algorithms and NP receiver operating characteristics (NP-ROC))
  • Citation: Tong, X., Feng, Y., and Li, J.J. (2018). Neyman-Pearson (NP) classification algorithms and NP receiver operating characteristics (NP-ROC). Science Advances 4(2):eaao1659.
• MSIQ (joint modeling of Multiple RNA-seq Samples for accurate Isoform Quantification)
  • Citation: Li, W.V., Zhao, A., Zhang, S., and Li, J.J. (2018). MSIQ: joint modeling of multiple RNA-seq samples for accurate isoform quantification. Annals of Applied Statistics 12(1):510-539.
• EpiAlign (an alignment-based bioinformatic tool for comparing chromatin state sequences)
  • Citation: Ge, X., Zhang, H., Xie, L., Li, W.V., Kwon, S.B., and Li, J.J. (2019). EpiAlign: an alignment-based bioinformatic tool for comparing chromatin state sequences. Nucleic Acids Research gkz287.
• scDesign (a statistical simulator for rational scRNA-seq experimental design)
  • Citation: Li, W.V. and Li, J.J. (2019). A statistical simulator scDesign for rational scRNA-seq experimental design. Bioinformatics 35(14):i41–i50.
• gR2 (generalized R squares measures)
  • Citation: Li, J.J., Tong, X., and Bickel, P.J. (2019). Generalized R2 measures for a mixture of bivariate linear dependences. arXiv:1811.09965.

Data

• Estimates of D. melanogaster and C. elegans gene expression in different developmental stages, tissues and cells (in FPKM units)
  • D. melanogaster gene expression estimates in 30 fly developmental stages (download)
  • D. melanogaster gene expression estimates in 29 fly tissues and 19 fly cell lines (download)
  • C. elegans gene expression estimates in 35 worm developmental stages (download)
  • C. elegans gene expression estimates in 4 worm tissues and 14 worm dissected cells (download)
  • Please cite the following paper in any research that uses the above data
    • Li, J.J., Huang, H., Bickel, P.B., and Brenner, S.E. (2014). Comparison of D. melanogaster and C. elegans developmental stages, tissues, and cells by modENCODE RNA-seq data. Genome Research 24(7):1086-1101.
  • For more details about the data, please refer to the section "Estimating gene expression in developmental stages and tissues/cells" in the Methods of the above paper ([html] [pdf]).

• Associated promoter and enhancer regions identified based on signals of three histone modification marks (H3K4me1, H3K4me3 and H3K27ac) in 16 human tissue and cell types (download)